(i) HeLa 229 cells (continuous cell line). HeLa229 cells grown on a 12-mm-diameter coverslip in a 1-dram (4-ml) vial shell were treated with 0 to 150 p,g of
HeLa 229 is a derivative of the parent HeLa line (ATCC CCL 2), but differs chiefly in its relative insusceptibility to polioviruses. Frozen cells of approximately the 60-70th passage of the parent HeLa line (ATCC CCL 2) were reactivated in March, 1959, subcultured for 8 passages, frozen in August, 1959, and again reactivated in April, 1960, for the American Type Culture Collection.
Several mutants of B. pertussis with Tn5 or Tn5 lac inserted into various vir-regulated genes were evaluate …. HeLa 229 cells infected with C. trachomatis L2/P ompA -GFP were exposed to increasing concentrations of azithromycin (0, 5, 50, and 100 ng/ml) starting from 0, 8, or 24 hpi. Cells were harvested at 40 hpi and subjected to the endpoint IFU assay to determine EB yields using immunofluorescence assays with an antibody specific to L2 MOMP. Updated November 01, 2018. HeLa cells are the first immortal human cell line. The cell line grew from a sample of cervical cancer cells taken from an African-American woman named Henrietta Lacks on February 8, 1951. The lab assistant responsible for the samples named cultures based on the first two letters of a patient's first and last name, thus the culture was dubbed HeLa.
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Several mutants of B. pertussis with Tn5 or Tn5 lac inserted into various vir-regulated genes were evaluate …. HeLa 229 cells infected with C. trachomatis L2/P ompA -GFP were exposed to increasing concentrations of azithromycin (0, 5, 50, and 100 ng/ml) starting from 0, 8, or 24 hpi. Cells were harvested at 40 hpi and subjected to the endpoint IFU assay to determine EB yields using immunofluorescence assays with an antibody specific to L2 MOMP. Updated November 01, 2018. HeLa cells are the first immortal human cell line. The cell line grew from a sample of cervical cancer cells taken from an African-American woman named Henrietta Lacks on February 8, 1951. The lab assistant responsible for the samples named cultures based on the first two letters of a patient's first and last name, thus the culture was dubbed HeLa.
HeLa cells have been infected with an array of diseases, from tuberculosis to salmonella, and have helped scientists understand that a normal human cell has 46 chromosomes, thus making genetic disorders easier to diagnose. It is easy to see why many have also named HeLa the mother of modern medicine.
HeLa 229 is a derivative of the parent HeLa line (ATCC CCL 2), but differs chiefly in its relative insusceptibility to polioviruses. Frozen cells of approximately the 60-70th passage of the parent HeLa line (ATCC CCL 2) were reactivated in March, 1959, subcultured for 8 passages, frozen in August, 1959, and again reactivated in April, 1960, for the American Type Culture Collection.
Apossible explanation for these results is that HeLa cells expressedan Fc-y receptor(s) that boundIgGantibodies coated onto the chlamydial surface and thereby abrogated the antibodies' neutralizing activity by facilitating parasite 2009-05-26 Further, to understand interdependence of IL-10 and iron homoeostasis (regulation), the levels of IL-10 were compared with iron-responsive GFP (green fluorescent protein) expression in HeLa-229 cells. 2020-12-06 1988-05-01 The inhibitor TABLE 4 EFFECT OF TIME FOR INDUCTION OF AN INHIBITOR OF POLIOVIRUS GROWTH BY BOUR IN HELA 229 CELLS Challenge Time--Hours* Polio TCIDso/tube N 70 7 0.7 24 I 4/4 4/4 4/4 N 4/4 4/4 4/4 72 I 4/4 2/4 0/4 N 3/3 3/3 4/4 120 I 0/4 0/4 0/4 4/4f 4/4 4/4 * Bour inoculum--104·8 ELD 60 /tube, incubate at 37°C before polio challenge, read tubes after 72 hours further incubation at 37°C t 0 HEK 293 cells are straightforward to grow in culture and to transfect.
HeLa 229 cells were infected with L2 or L2R at a multiplicity of infection of 3. Lysates of chlamydia-infected HeLa 229 cells were prepared in RIPA buffer or 8 M urea at various times postinfection.
HeLa (/ ˈ h iː l ɑː /; also Hela or hela) is an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line is derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a 31-year-old African-American mother of five, who died of cancer on October 4, 1951. The genotype of the patient Henrietta Lacks from whose cervical carcinoma the HeLa cell was derived was deduced from the phenotypes of her husband and children, and from studies of the HeLa cell.
2. Omslag. [Developmental cell (Online)]; Developmental cell [Elektronisk resurs]; 2001-; E-tidskrift. Tabell 4 Personer med någon form av äldreomsorg efter kön och ålder, hela staden 229. Hela Staden. 5085.
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LIBRIS sökning: Molecular biology of the cell. Läs hela. 2. Omslag.
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